Óleo de copaíba associado a ozonioterapia no tratamento do melanoma experimental em camundongos

Abstract

Melanoma is one of the most severe types of skin cancer due to its metastatic potential, being common in Brazil and worldwide. The present study investigated the therapeutic action of copaiba oil associated with ozonotherapy in experimentally induced melanoma. Copaiba oil was used in its isolated form, polyaltic acid. Both treatments were chosen for their anti-inflammatory and healing properties. The experiment conducted with 32 Balb/c strain mice, both males and females, lasted 26 days.The animals were inoculated with B16-F10 tumor cells subcutaneously and divided into five groups treated daily: group 1, control received only tumor injection; group 2, received ozonotherapy; group 3, received polyaltic acid; group 4, received the combination of both treatments. Ozonotherapy was applied via subcutaneous injection, while polyaltic acid was administered by gavage. The results were evaluat by tumor measurement, histopathological analysis of organs and tumor, and leukocyte count in peritoneal lavage. The combination of polyaltic acid and ozonotherapy demonstrated antitumor efficacy, reducing tumor volume and decreasing leukocytes in the group treated only with ozonotherapy. However, no reduction in neutrophil recruitment was observed in the groups treated with polyaltic acid and no metastases were found. The study concludes that the combination of these treatments holds potential, but clinical investigations are needed to establish more suitable protocols, considering the type of cancer and the clinical characteristics of oncological patients.